TISSUE CULTURE TECHNOLOGY

TRAINING COURSE

Course outline

General introduction

Media Preparation

              Components of tissue culture medium

              Preparation and sterilization of media

Sterilization Methods

Sterilization of media and media components, glassware, instruments, work area etc.

Laminar flow/Glove box

              Tissue culture work areas

Initiation

            Plant selection and preparation

              Establishment of clean cultures

Multiplication

              Subculturing of plants

Hardening

            Transfer of plantlets from growth room to shade house

Quality control

            Monitoring plants for pests, disease, contamination and mutation

SCHEDULE OF ACTIVITIES

Day 1

8:30-9:00am                 Registration

9:00-9:30                      Opening ceremony

9:30-10:30                    Lecture: Introduction to tissue culture

10:30-11:00                  BREAK

11:00-12:00                  Lecture: Media preparation, sterilization methods

12:00-1:00                    LUNCH

1:00-4:00                      Practical: Preparation and sterilization of media,

                                      glassware, equipment

Day 2

9:00-10:30                    Lecture: Laminar flow/glove box, sterile technique,

                                      initiation, multiplication

10:30-11:00                  BREAK

11:00-12:00                  Practical: Initiation of African violet

12:00-1:00                    LUNCH

1:00-4:00                      Practical: Initiation of African violet, subculturing of

                                      selected  plants

Day 3

9:00-10:30                     Lecture: Hardening of tissue culture plantlets,

                            quality control

10:30-11:00                   BREAK

11:00-12:00                   Lecture: Quality control

12:00-1:00                     LUNCH

1:00-4:00                       Practical: Subculture of selected plants

Day 4

9:00-10:30                     Examination and Course evaluation

10:30-11:00                   BREAK

11:00-12:00                   Practical: Hardening

12:00-1:00                     LUNCH

1:00-2:00                       Discussion

2:00-3:00                       Closing ceremony